Fungicide Assay by Spore Germination in Shaker Flasks

Fungicide assay by spore germination in shaker flasks.

Since the introduction in 1933 (Kluyver and Perquin, 1933) of the shaker flask method of culturing fungi this technique has found widespread use in fungus physiology (Foster, 1949). It was used for spore germination studies by Davies et al. (1948) and Mandels and Norton (1948). The measurement of antifungal activity, however, has been accomplished by the more conventional methods making use of ...

Ecophysiology of Development: Spore Germination

Ecophysiology of Development: Spore Germination

Tissue Sap on Spore Germination

Bacillus megaterium spore germination is influenced by inoculum size.

AIMS The effect of spore density on the germination (time-to-germination, percent germination) of Bacillus megaterium spores on tryptic soy agar was determined using direct microscopic observation. METHODS AND RESULTS Inoculum size varied from approximately 10(3) to 10(8) cfu ml(-1) in a medium where pH=7 and the sodium chloride concentration was 0.5% w/v. Inoculum size was measured by global...